Soligenix Provides Regulatory Update on HyBryte™

On April 14, 2023 Soligenix, Inc. (Nasdaq: SNGX) (Soligenix or the Company), a late-stage biopharmaceutical company focused on developing and commercializing products to treat rare diseases where there is an unmet medical need, reported that the Company convened a Type A Meeting with the United States (U.S.) Food and Drug Administration (FDA) (Press release, Soligenix, APR 14, 2023, View Source [SID1234630094]). During the Type A Meeting, representatives of the Company and the FDA discussed the contents of a refusal to file (RTF) letter previously issued by the FDA regarding the Company’s new drug application (NDA) for HyBryte (synthetic hypericin sodium) in the treatment of early stage cutaneous T-cell lymphoma (CTCL), a rare cancer, where it successfully demonstrated statistically significant results in a Phase 3 clinical trial (Study HPN-CTCL-01; also referred to as the FLASH study).

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In order to accept an NDA filing for HyBryte, the FDA is requiring positive results from a second clinical study in addition to the Phase 3, randomized, double-blind, placebo-controlled FLASH study previously conducted in this orphan indication. The FDA indicated that it is open to engaging in protocol discussions regarding the second clinical study. Based on the feedback, the Company has decided to collaboratively engage in discussions with the FDA in order to define the protocol and evaluate the feasibility of conducting the additional clinical trial.

"While we are very disappointed by this delay, Soligenix and its clinical investigators remain committed to working with the FDA and advancing HyBryte to market for patients suffering with CTCL," stated Christopher J. Schaber, PhD, President and Chief Executive Officer of Soligenix. "The Phase 3 FLASH study was the largest double-blind, randomized, placebo-controlled clinical trial ever conducted in the CTCL population. While we are surprised that the FDA did not accept our submitted NDA for filing and review, it was very clear that the FDA’s thinking has evolved in evaluating CTCL therapies since our initial protocol discussions on Study HPN-CTCL-01 and that another Phase 3 study will be required to support an NDA for HyBryte. During the Type A meeting, we discussed elements of protocol design for the additional confirmatory study and look forward to collaborating with the FDA to advance these discussions as quickly as possible to have a reasonable study design to meet the FDA’s requirements."

About HyBryte

HyBryte (research name SGX301) is a novel, first-in-class, photodynamic therapy utilizing safe, visible light for activation. The active ingredient in HyBryte is synthetic hypericin sodium, a potent photosensitizer that is topically applied to skin lesions that is taken up by the malignant T-cells, and then activated by visible light approximately 24 hours later. The use of visible light in the red-yellow spectrum has the advantage of penetrating more deeply into the skin (much more so than ultraviolet light) and therefore potentially treating deeper skin disease and thicker plaques and lesions. This treatment approach avoids the risk of secondary malignancies (including melanoma) inherent with the frequently employed DNA-damaging drugs and other phototherapies that are dependent on ultraviolet exposure. Combined with photoactivation, hypericin has demonstrated significant anti-proliferative effects on activated normal human lymphoid cells and inhibited growth of malignant T-cells isolated from CTCL patients. In a published Phase 2 clinical study in CTCL, patients experienced a statistically significant (p=0.04) improvement with topical hypericin treatment whereas the placebo was ineffective. HyBryte has received orphan drug and fast track designations from the FDA, as well as orphan designation from the European Medicines Agency (EMA).

The recently published Phase 3 FLASH (Fluorescent Light Activated Synthetic Hypericin) study enrolled a total of 169 patients (166 evaluable) with Stage IA, IB or IIA CTCL. The trial consisted of three treatment cycles. Treatments were administered twice weekly for the first 6 weeks and treatment response was determined at the end of the 8th week of each cycle. In the first double-blind treatment cycle, 116 patients received HyBryte treatment (0.25% synthetic hypericin) and 50 received placebo treatment of their index lesions. A total of 16% of the patients receiving HyBryte achieved at least a 50% reduction in their lesions (graded using a standard measurement of dermatologic lesions, the CAILS score) compared to only 4% of patients in the placebo group at 8 weeks (p=0.04) during the first treatment cycle (primary endpoint). HyBryte treatment in the first cycle was safe and well tolerated.

In the second open-label treatment cycle (Cycle 2), all patients received HyBryte treatment of their index lesions. Evaluation of 155 patients in this cycle (110 receiving 12 weeks of HyBryte treatment and 45 receiving 6 weeks of placebo treatment followed by 6 weeks of HyBryte treatment), demonstrated that the response rate among the 12-week treatment group was 40% (p<0.0001 vs the placebo treatment rate in Cycle 1). Comparison of the 12-week and 6-week treatment groups also revealed a statistically significant improvement (p<0.0001) between the two groups, indicating that continued treatment results in better outcomes. HyBryte continued to be safe and well tolerated. Additional analyses also indicated that HyBryte is equally effective in treating both plaque (response 42%, p<0.0001 relative to placebo treatment in Cycle 1) and patch (response 37%, p=0.0009 relative to placebo treatment in Cycle 1) lesions of CTCL, a particularly relevant finding given the historical difficulty in treating plaque lesions in particular.

The third (optional) treatment cycle (Cycle 3) was focused on safety and all patients could elect to receive HyBryte treatment of all their lesions. Of note, 66% of patients elected to continue with this optional compassionate use / safety cycle of the study. Of the subset of patients that received HyBryte throughout all 3 cycles of treatment, 49% of them demonstrated a positive treatment response (p<0.0001 vs patients receiving placebo in Cycle 1). Moreover, in a subset of patients evaluated in this cycle, it was demonstrated that HyBryte is not systemically available, consistent with the general safety of this topical product observed to date. At the end of Cycle 3, HyBryte continued to be well tolerated despite extended and increased use of the product to treat multiple lesions.

Overall safety of HyBryte is a critical attribute of this treatment and was monitored throughout the three treatment cycles (Cycles 1, 2 and 3) and the 6-month follow-up period. HyBryte’s mechanism of action is not associated with DNA damage, making it a safer alternative than currently available therapies, all of which are associated with significant and sometimes fatal, side effects. Predominantly these include the risk of melanoma and other malignancies, as well as the risk of significant skin damage and premature skin aging. Currently available treatments are only approved in the context of previous treatment failure with other modalities and there is no approved front-line therapy available. Within this landscape, treatment of CTCL is strongly motivated by the safety risk of each product. HyBryte potentially represents the safest available efficacious treatment for CTCL. With very limited systemic absorption, a compound that is not mutagenic and a light source that is not carcinogenic, there is no evidence to date of any potential safety issues.

The Phase 3 CTCL clinical study was partially funded by the National Cancer Institute via a Phase II SBIR grant (#1R44CA210848-01A1) awarded to Soligenix, Inc. In addition, the FDA awarded an Orphan Products Development grant to support the evaluation of HyBryte for expanded treatment in patients with early-stage CTCL, including in the home use setting. The grant, totaling $2.6 million over 4 years, was awarded to a prestigious academic institution that was a leading enroller in the Phase 3 FLASH study.

About Cutaneous T-Cell Lymphoma (CTCL)

CTCL is a class of non-Hodgkin’s lymphoma (NHL), a type of cancer of the white blood cells that are an integral part of the immune system. Unlike most NHLs which generally involve B-cell lymphocytes (involved in producing antibodies), CTCL is caused by an expansion of malignant T-cell lymphocytes (involved in cell-mediated immunity) normally programmed to migrate to the skin. These malignant cells migrate to the skin where they form various lesions, typically beginning as patches and may progress to raised plaques and tumors. Mortality is related to the stage of CTCL, with median survival generally ranging from about 12 years in the early stages to only 2.5 years when the disease has advanced. There is currently no cure for CTCL. Typically, CTCL lesions are treated and regress but usually return either in the same part of the body or in new areas.

CTCL constitutes a rare group of NHLs, occurring in about 4% of the approximate 700,000 individuals living with the disease. It is estimated, based upon review of historic published studies and reports and an interpolation of data on the incidence of CTCL that it affects over 25,000 individuals in the U.S., with approximately 3,000 new cases seen annually.

QIAGEN showcases latest technologies to advance cancer research at AACR Annual Meeting 2023

On April 14, 2023 QIAGEN (NYSE: QGEN; Frankfurt Prime Standard: QIA) reported the launch of QIAseq Targeted cfDNA Ultra Panels that will enable researchers studying cancer and other diseases to turn cell-free DNA (cfDNA) liquid-biopsy samples into libraries ready for next-generation sequencing (NGS) in less than eight hours (Press release, Qiagen, APR 14, 2023, View Source [SID1234630093]).

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The new kit adds another innovation to the QIAseq Targeted DNA product portfolio. It will be one of the central features of QIAGEN’s life-science offering at the 2023 annual meeting of the American Association for Cancer Research (AACR) (Free AACR Whitepaper) in Orlando, Florida, from April 14 to 19, 2023.

Liquid biopsy centered on cfDNA has become a vital tool in the diagnosis, outcome prognosis and treatment monitoring of cancer and other diseases. Carried by blood and other body fluids, cfDNA eliminates the need for surgery to take tissue samples. But it often carries disease-relevant variants in low concentrations, which means researchers demand extremely sensitive and reliable tools.

The QIAseq Targeted cfDNA Ultra Panels meet this requirement as they enable reliable detection of somatic genetic variants in challenging detection scenarios as low as 0.1% variant allele frequency (VAF) by enhanced chemistry, reduced enzymatic error rates, and an optimized bioinformatics pipeline.

"QIAGEN is dedicated to driving innovation in liquid biopsy technology to enhance cancer research and improve patient outcomes. With the launch of our QIAseq Targeted cfDNA Ultra Panels, researchers can now rapidly and accurately detect somatic genetic variants at low concentrations, providing a valuable tool for investigation of cancer and other diseases," said Dr. Thomas Schweins, Senior Vice President and Head of QIAGEN’s Life Sciences Business Area. "We are delighted to engage with experts at AACR (Free AACR Whitepaper) 2023 and demonstrate our many contributions to fighting cancer."

The proven ability of QIAGEN technology to detect tiny traces cfDNA variants will feature in the AACR (Free AACR Whitepaper)’s Spotlight Theater Talks from 10-11 a.m. on April 17. Marzia Del Re from the University of Pisa, Italy, will present her study of QIAGEN’s QIAcuity nanoplate-based digital PCR and other systems. "Our data show QIAcuity has a higher sensitivity than droplet digital PCR," she said. "This allows the detection of a larger number of mutated patients, even with low cfDNA abundance."

The QIAcuity digital PCR system enables researchers to detect and quantify DNA and RNA targets with high precision and sensitivity, allowing for reliable analysis of rare and difficult-to-detect targets for a wide range of applications. The instruments integrate partitioning, thermocycling and imaging into one workflow, cutting processing times to only two hours.

QIAGEN will also add new pan-cancer panels to its digital PCR portfolio. These panels offer a cutting-edge solution for the investigation of the most important cancer-related genes and will be available to customers starting in fall 2023. With a focus on hallmark mutations within specific genes, these assays enable researchers to investigate samples in multiplex reactions, allowing for faster and more efficient analysis. The panels are suitable for a range of applications, including biomarker validation, orthogonal validation of next-generation sequencing, resistance monitoring, drug monitoring, and tumor characterization.

QIAcuity’s extremely reliable mutation detection can be coupled with the easy sample processing of QIAGEN’s EZ2 Connect system. The platform for fully automated and convenient sample processing purifies DNA and RNA from various sample types using prefilled reagent-cartridges, contributing to optimized workflows and greater lab productivity. QIAcuity ensures fast and sensitive ultra-low mutation detection – a ground-breaking end-to-end combination for cancer researchers.

QIAGEN is looking forward to hosting AACR (Free AACR Whitepaper) attendees at booth #753 in Orlando’s Orange County Convention Center. It will here unveil QIAcube Connect Red, a limited edition of QIAGEN’s gold-standard automated device for DNA, RNA and protein sample processing. Over 10,000 QIAcube instruments with blue trims have been installed – but there will always only be 100 devices with a red door-trim.

Learn more about QIAGEN’s commitment to fighting cancer at www.qiagen.com/conquer-cancer.

Onxeo will publish its full-year results on April 21, 2023

On April 14, 2023 Onxeo S.A. (Euronext Growth Paris: ALONX), a clinical-stage biotechnology company specializing in the development of innovative drugs targeting tumor DNA Damage Response (DDR) and driver oncogenes, reported new date for the publication of its 2022 full-year results (Press release, Onxeo, APR 14, 2023, View Source [SID1234630092]).

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The publication of the 2022 full-year results is postponed to April 21, 2023, after market close.

NuCana Presents Data at the AACR 2023 Annual Meeting Highlighting Novel NUC-7738 Mechanisms of Action

On April 14, 2023 NuCana plc (NASDAQ: NCNA) announced two posters to be presented at the American Association of Cancer Research (AACR) (Free AACR Whitepaper) Annual Meeting being held from April 14-19, 2023 (Press release, Nucana BioPharmaceuticals, APR 14, 2023, View Source [SID1234630089]).

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Title: NUC-7738 causes reduction of soluble and exosome-associated PD-L1 in melanoma cell lines and patients
Presentation date and time: April 19th from 9.00am – 12.30pm
Abstract number: 5962

This study investigates the dynamic between NUC-7738 and the secreted forms of PD-L1 (soluble PD-L1 and exosomal PD-L1) both in a melanoma cell line and in patients treated with NUC-7738. Data from this study demonstrate that NUC-7738 reduced mRNA and protein levels of soluble PD-L1 in a melanoma cell line and that patients treated with NUC-7738 had reduced levels of exosomal PD-L1 compared to pre-treatment levels. These data indicate that NUC-7738 has the potential to act as an immune sensitizer and as an effective combination partner for PD-L1 pathway inhibitors. NUC-7738 is currently being studied in combination with pembrolizumab for patients with advanced solid tumors in the Phase 2 part of the NuTide:701 study.

Title: NUC-7738 promotes alternative polyadenylation site usage and reduces glutaminase GAC isoform
Presentation date and time: April 16th from 1.30pm – 5.00pm
Abstract number: 277

This study investigates the effect of NUC-7738 on isoforms of glutaminase 1. Glutaminase is often upregulated in cancer cells. Additionally, the GAC isoform drives more metabolically active cancers and has been associated with poorer patient outcomes. NUC-7738 reduced the expression of GAC in renal and pancreatic cancer cell lines in both hypoxic and normoxic conditions. Furthermore, tissue from patients with kidney cancer that was treated with NUC-7738 ex vivo demonstrated reduced levels of GAC. These data highlight a potentially meaningful anti-cancer strategy for NUC-7738 in metabolically active tumors that are able to sustain high rates of growth and proliferation in unfavorable conditions, including hypoxia.

Hugh S. Griffith, NuCana’s Founder and Chief Executive Officer said: "We are excited with these data as we believe they demonstrate NUC-7738’s multi-faceted mechanisms of action and are highly supportive of our clinical development strategy. We are currently investigating NUC-7738 in combination with pembrolizumab in the Phase 2 part of the NuTide:701 study and look forward to sharing data from this study in 2023."

MEI PHARMA ANNOUNCES 1-FOR-20 REVERSE STOCK SPLIT

On April 13, 2023 MEI Pharma, Inc. (Nasdaq: MEIP), a clinical-stage pharmaceutical company focused on advancing new therapies for cancer, reported that its board of directors approved a 1-for-20 reverse stock split (Press release, MEI Pharma, APR 14, 2023, View Source [SID1234630087]). The Company’s common stock will open for trading on the Nasdaq Capital Market on Monday, April 17, 2023 on a split-adjusted basis under the current trading symbol "MEIP." The reverse stock split was approved by MEI’s stockholders on January 5, 2023, and is intended to increase the per share trading price of the Company’s common stock to enable the Company to satisfy the minimum bid price requirement for continued listing on Nasdaq.

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The 1-for-20 reverse stock split will automatically convert 20 current shares of MEI’s common stock into one new share of common stock. No fractional shares will be issued in connection with the reverse stock split. Stockholders who would otherwise hold a fractional share of MEI’s common stock will receive a cash payment in lieu thereof, at a price equal to the fraction to which the stockholder would otherwise be entitled, multiplied by the closing price of MEI’s common stock on Nasdaq on Friday, April 14, 2023. The reverse split will reduce the number of shares of outstanding common stock from approximately 133,260,865 shares to approximately 6,663,043 shares. Proportional adjustments also will be made to the exercise prices of MEI’s outstanding stock options and warrants, and to the number of shares issued and issuable under MEI’s stock incentive plan.

Computershare Trust Company, N. A. ("Computershare") will act as the transfer agent for the reverse stock split. Stockholders holding their shares electronically in book-entry form are not required to take any action to receive post-split shares. Stockholders owning shares through a bank, broker or other nominee will have their positions automatically adjusted to reflect the reverse stock split, subject to brokers’ particular processes, and will not be required to take any action in connection with the reverse stock split. For those stockholders holding physical stock certificates, Computershare will send instructions for exchanging those certificates for shares held electronically in book-entry form or for new certificates, in either case representing the post-split number of shares.

In connection with the reverse stock split, the Company’s CUSIP number will change to 55279B301 on Monday, April 17, 2023.